2023 Summer Undergraduate Research Program (SURP) Symposium
Location
ScholarSpace, Rod Library, University of Northern Iowa
Presentation Type
Open Access Poster Presentation
Document Type
poster
Keywords
Barley--Drought tolerance; Spermidine; Mosaic viruses;
Abstract
Polyamines are low molecular weight aliphatic nitrogenous compounds with two or more amino groups (Liu et al., 2015). Polyamines are found in all organisms. They play diverse roles in growth and development as well as environmental stress response. One of the major polyamines in plants is spermidine. Spermidine increases grain filling by increasing hormones and enzymes in sucrose biosynthesis (Luo et al., 2019). Expression of genes in the spermidine biosynthesis pathway increases during drought in barley (Abebe et al., 2010). However, the role of spermidine biosynthesis for drought tolerance has not been systematically investigated in barley. In the past, we used barley stripe mosaic virus (BSMV) to silence expression of spermidine synthase (SPDS), a key enzyme in spermidine biosynthesis. BSMV is an aggressive virus and made barley plants sick and difficult to study drought response. In this research, we used a less aggressive foxtail mosaic virus (FoMV) to suppress SPDS and determine the response of barley to drought.
Start Date
28-7-2023 11:00 AM
End Date
28-7-2023 1:30 PM
Event Host
Summer Undergraduate Research Program, University of Northern Iowa
Faculty Advisor
Tilahun Abebe
Department
Department of Biology
Copyright
©2023 Ammar A. Shaikh and Tilahun Abebe
File Format
application/pdf
Recommended Citation
Shaikh, Ammar A. and Abebe, Tilahun, "Functional Analysis of Spermidine Synthase (SPDS) using Virus-Induced Gene Silencing in Barley" (2023). Summer Undergraduate Research Program (SURP) Symposium. 27.
https://scholarworks.uni.edu/surp/2023/all/27
Functional Analysis of Spermidine Synthase (SPDS) using Virus-Induced Gene Silencing in Barley
ScholarSpace, Rod Library, University of Northern Iowa
Polyamines are low molecular weight aliphatic nitrogenous compounds with two or more amino groups (Liu et al., 2015). Polyamines are found in all organisms. They play diverse roles in growth and development as well as environmental stress response. One of the major polyamines in plants is spermidine. Spermidine increases grain filling by increasing hormones and enzymes in sucrose biosynthesis (Luo et al., 2019). Expression of genes in the spermidine biosynthesis pathway increases during drought in barley (Abebe et al., 2010). However, the role of spermidine biosynthesis for drought tolerance has not been systematically investigated in barley. In the past, we used barley stripe mosaic virus (BSMV) to silence expression of spermidine synthase (SPDS), a key enzyme in spermidine biosynthesis. BSMV is an aggressive virus and made barley plants sick and difficult to study drought response. In this research, we used a less aggressive foxtail mosaic virus (FoMV) to suppress SPDS and determine the response of barley to drought.
