Dissertations and Theses @ UNI
Availability
Open Access Thesis
Keywords
Arabidopsis thaliana; Callus (Botany);
Abstract
An investigation of the effects of factorial combinations of race, explant, induction medium and age on chromosomal stability and regenerative ability of Arabidopsis thaliana callus was performed. Regenerative ability of primary and subcultured callus from all treatments was also examined. Somatic counts were performed on a sample of calluses from factorial combinations of two explants (anther and seedling) and two races (WS and HM) induced on three induction media (DBM-1, ACM-1 and PG-1) and subcultured onto maintenance medium (DBM-2-M) after 30, 60 and 90 days. At each subculture, the mean chromosome number for each factorial treatment was close to the expected haploid or diploid chromosome number, indicating that true haploid and diploid callus was induced and maintained. Racial effects on chromosomal stability were absent throughout this study. Significant effects due to the use of the different induction media, although present in aging callus, were not detected in primary callus. Substantial aneuploidy was observed throughout this study in all callus types examined. The distribution of aneuploid cells was skewed to the right of the expected haploid chromosome number (5) in anther callus; for seedling callus this distribution was skewed to the left of the expected diploid chromosome number (10). These opposing distributions were observed at each subculture. These trends are consistent with them hypothesis that mitotic non-disjunction and chromosome lagging were operating simultaneously to cause the observed aneuploidy. Polyploidization occurred more frequently in anther callus than in callus derived from seedlings. Most of the anther-derived polyploids were diploid. These diploids may have arisen by mitotic failure resulting in doubled-haploids or they may represent maternal diploid cells included in the predominantly haploid callus. Regeneration frequencies in primary callus were extremely low, bud did not decline in subcultured callus. Callus derived from race WS regenerated more frequently at all three subcultures, in comparison to race HM. Regeneration medium, DBM-2-S, produced a much higher average frequency of regenerants in comparison to PG-3. Because the frequency of regeneration was so low, no conclusions could be made about the relationship of aneuploidy to regenerative ability.
Year of Submission
1979
Degree Name
Master of Arts
Department
Department of Biology
First Advisor
Jean A. Amos
Second Advisor
Alan R. Orr
Third Advisor
Daryl D. Smith
Date Original
1979
Object Description
1 PDF file (127 leaves)
Copyright
©1979 Kenneth A. Feldman
Language
en
File Format
application/pdf
Recommended Citation
Feldman, Kenneth A., "Chromosome Stability and Regenerative Potential of Anther and Seedling Callus of Arabidopsis thaliana: Effect of Age, Race and Induction Medium" (1979). Dissertations and Theses @ UNI. 2622.
https://scholarworks.uni.edu/etd/2622
Comments
If you are the rightful copyright holder of this thesis and wish to have it removed from the Open Access Collection, please submit a request to scholarworks@uni.edu and include clear identification of the work, preferably with URL.