2018 Research in the Capitol

Presentation Type

Open Access Poster Presentation

Keywords

Adenosine triphosphate--Physiological effect; Epithelial cells--Diseases; Actin--Effect of chemicals on;

Abstract

Epithelial cells lining secretory units and ducts of bovine mammary glands perform an important role in regulating movement of various macromolecules and whole cells during normal lactation and mastitis. During mastitis, host and bacterial produced substances can affect the “barrier” function of epithelial monolayers. One potential component is adenosine triphosphate (ATP). ATP likely interacts with P2X7, a purinergic receptor, in mediating some effects associated with mastitis. Bovine mammary gland epithelial cell line, Mac-T cells, were examined for cytoskeletal changes as result of P2X7 interactions. Actin cytoskeletons were stained with phalloidin and effects were examined by fluorescent microscopy. Observable increase in actin fibril size was noted in ATP treated cells, and not seen in cells treated with P2X7 inhibitors prior to ATP exposure. Results indicate the possibility of ATP modulating epithelial cell function in bovine mammary glands, affecting the barrier function epithelial cells normally provide, through interaction with the P2X7 receptor.

Start Date

3-4-2018 11:30 AM

End Date

3-4-2018 1:30 PM

Event Host

University Honors Programs, Iowa Regent Universities

Faculty Advisor

David McClenahan

Department

Department of Biology

File Format

application/pdf

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Apr 3rd, 11:30 AM Apr 3rd, 1:30 PM

Extracellular ATP Effects on Intracellular Actin Fibrils' Location and Characteristics [Poster]

Epithelial cells lining secretory units and ducts of bovine mammary glands perform an important role in regulating movement of various macromolecules and whole cells during normal lactation and mastitis. During mastitis, host and bacterial produced substances can affect the “barrier” function of epithelial monolayers. One potential component is adenosine triphosphate (ATP). ATP likely interacts with P2X7, a purinergic receptor, in mediating some effects associated with mastitis. Bovine mammary gland epithelial cell line, Mac-T cells, were examined for cytoskeletal changes as result of P2X7 interactions. Actin cytoskeletons were stained with phalloidin and effects were examined by fluorescent microscopy. Observable increase in actin fibril size was noted in ATP treated cells, and not seen in cells treated with P2X7 inhibitors prior to ATP exposure. Results indicate the possibility of ATP modulating epithelial cell function in bovine mammary glands, affecting the barrier function epithelial cells normally provide, through interaction with the P2X7 receptor.