Open Access Honors Program Thesis
David McClenahan, Honors Thesis Advisor
Adenosine triphosphate--Physiological effect; Actin--Effect of chemicals on; Mastitis;
Epithelial cells lining secretory units and ducts of bovine mammary glands perform an important role in regulating movement of various macromolecules and whole cells during normal lactation and mastitis. During mastitis, host-and bacteria-produced substances can affect the “barrier” function of epithelial monolayers. One potential component is adenosine triphosphate (ATP). ATP likely interacts with P2X7, a purinergic receptor, in mediating some effects associated with mastitis including changes in cell permeability. The bovine mammary gland epithelial cell line, Mac-T cells, were examined for cytoskeletal changes as result of P2X7 interactions. Actin cytoskeletons were stained with phalloidin and effects were examined by fluorescent microscopy. Observable increase in actin fibril size was noted in ATP treated cells. Results indicate the possibility of ATP modulating epithelial cell function in bovine mammary glands affecting the barrier function epithelial cells normally provide, through interaction with the P2X7 receptor.
Year of Submission
Department of Biology
McNair Scholars Program at UNI
University Honors Designation
A thesis submitted in partial fulfillment of the requirements for the designation University Honors
1 PDF file (30 pages)
©2018 Dianna Huisman
Huisman, Dianna, "Effect of extracellular ATP on cellular actin fibrils' location and characteristics" (2018). Honors Program Theses. 322.