Selective Recovery of β-Galactosidase With Charged Fusion Tails Using Ion-Exchange Membranes

Authors

Erika A. Thiem, Iowa State University
Meng H. Heng, Iowa State Universtiy

Document Type

Research

Keywords

fusion proteins, purification fusions, protein purification, ion-exchange, β-galactosidase

Abstract

We explored the feasibility of attaching charged tails to a target protein, in this case β-galactosidase, for selective recovery. In this process, an ion-exchange membrane was used for selective binding and release of β-galactosidase with the attached purification fusions. Strength of binding and purity of eluate increased with increasing tail length. In addition, activity yield was improved with the implementation of an intermediate partial elution recycle procedure.

Publication Date

June 1994

Journal Title

Journal of the Iowa Academy of Science

Volume

101

Issue

2

First Page

66

Last Page

69

Copyright

© Copyright 1994 by the Iowa Academy of Science, Inc.

Language

EN

File Format

application/pdf

Recommended Citation

Thiem, Erika A. and Heng, Meng H. (1994) "Selective Recovery of β-Galactosidase With Charged Fusion Tails Using Ion-Exchange Membranes," Journal of the Iowa Academy of Science: JIAS, 101(2), 66-69.
Available at: https://scholarworks.uni.edu/jias/vol101/iss2/10