Dissertations and Theses @ UNI
Availability
Open Access Thesis
Keywords
Time-of-flight mass spectrometry;
Abstract
Detector saturation and the lack of mass spectrometry-mass spectrometry (MSMS) correlation techniques has limited the utility of time-of-flight mass spectrometry (TOF-MS). This research focused on solving these problems with the use of an electrostatic particle guide and a novel two-dimensional correlation technique. Microchannel plate detectors that are used with TOF-MS have been shown to be susceptible to saturation due to high ion influxes. Matrix assisted laser desorption ionization (MALDI), as used for this research, produces a large number of low molecular weight ions that cause excessive detector saturation. This problem was addressed with the development of a bipolar pulsed electrostatic particle guide (EPG). The EPG is a wire that traverses the length of the flight tube with switchable positive or negative voltage applied to it to either deflect or transport ions to the detector. The deflection of the unwanted portion of the mass spectrum lead to less detector saturation and improved instrument sensitivity. As molecules of larger mass are studied, more information is needed from the mass spectrometer to elucidate the structure of the compound. There are several fragments in the mass spectrum associated with the various side chains, rings, and backbone of a large biomolecule, making it hard to determine the overall structure without a correlation technique to provide more information as to the origin of the fragments. A technique has been proposed to address this problem using a linear TOF-MS equipped with a metastable ion repeller acting as a broad band kinetic energy analyzer to allow MS-MS correlation studies to be performed. This technique should work well due the simplicity of the correlation, but inconsistent "shot to shot" reproducibility of peak intensities within the mass spectrum will have to be addressed for the technique to be practical.
Year of Submission
1994
Degree Name
Master of Arts
Department
Department of Chemistry
First Advisor
Curtiss D. Hanson
Second Advisor
Ira M. Simet
Third Advisor
Bart L. Bergquist
Date Original
1994
Object Description
1 PDF file (93 leaves)
Copyright
©1994 Craig Lavern Just
Language
en
File Format
application/pdf
Recommended Citation
Just, Craig Lavern, "Enhancement of a Time-Of-Flight Mass Spectrometer Using a Bipolar Pulsed Electrostatic Particle Guide and a Novel MS-MS Correlation Technique" (1994). Dissertations and Theses @ UNI. 3100.
https://scholarworks.uni.edu/etd/3100
Comments
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