Dissertations and Theses @ UNI

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Open Access Thesis

Keywords

Calcification; Calciphylaxis; Rats--Physiology;

Abstract

Calciphylactic nephrocalcinosis was induced in female Sprague Dawley rats by treatment with dihydrotachysterol (0.5 mg/100g body weight) and thallous acetate (2 mg/100 g body weight). A calcium-phosphatephospholipid complex was isolated from rat kidneys exhibiting calciphylactic nephrocalcinosis and from untreated controls. There was a statistically significant greater quantity of complex extractable after calciphylaxis was induced. An average of 8.0% of the total phospholipid extracted from the tissue was present in complexed form in treated animals and 3.7 % in control animals. The phospholipid component of the complex was identified as phosphatidyl serine. The ratio of calcium to phosphate was less than 0.5, which is similar to the ratio reported in the literature for a calcium-phosphatephospholipid complex isolated from tumors but dissimilar to the ratio of unity reported for a complex isolated from normally calcified tissues. The significance of the calcium-phosphate-phospholipid complex in calcifying tissue is discussed and a possible mechanism for calciphylactic nephrocalcinosis is presented.

Year of Submission

1982

Degree Name

Master of Arts

Department

Department of Biology

First Advisor

Robert J. Simpson

Second Advisor

Robert Gross

Third Advisor

Alan R. Orr

Comments

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Date Original

1982

Object Description

1 PDF file (133 leaves)

Language

en

File Format

application/pdf

Included in

Biology Commons

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