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Open Access Thesis

Keywords

Cruciferae--Phylogeny; Cruciferae; Academic theses;

Abstract

The taxonomy of Physaria has continually been in a state of flux, especially certain groups of species within the genus. The Physaria reediana species complex (sensu Rollins) is an example of one of these problematic groups. The systematics of the Physaria reediana species complex was examined by a combination of techniques. A Bayesian analysis of 71 aligned sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) provided a fairly well-supported phylogeny (mean posterior probability per node: 0.5429) of a portion of the genus, which included the. species in the complex. Recent nodes showed the highest statistical support, while deeper nodes were short and showed low posterior probabilities. Microsatellite data from inter simple sequence repeats (ISSR) proved uninformative. Based on the ITS sequence phylogeny, the members of the P. reediana species complex (as formerly defined) are not as closely related as previously suspected. Morphological characters, as well as biogeographical information, were compared to the resultant phylogeny and relevant species boundaries were defined according to the phylogenetic species concept. Several taxonomic changes were necessary. Physaria spatulata comb. nov. is no longer recognized as a subspecific taxon of P. reediana. However, Physaria reediana subsp. curvipes st. nov. is described as a subspecies of P. reediana due to the combination of evidence. Physaria pycnantha sp. nov. is clearly distinct from P. nelsonii, under which it was formerly circumscribed. Physaria pachyphylla sp. nov. and Physaria eriocarpa sp. nov., narrow endemics from Montana, were confirmed to be new species as a result of this study. Several factors (low statistical support for deeper branches, limited geographic distributions for some taxa, and morphological similarities) are indicative of a relatively recent radiation of, not only members under study here, but the genus as a whole. Achieving a coherent and useful taxonomy of this group of species came about only with the synthesis of the molecular, morphological, and biogeographical data.

Year of Submission

2005

Degree Name

Master of Science

Department

Department of Biology

First Advisor

Stephen O'Kane

Second Advisor

James W. Demastes

Third Advisor

James E. Jurgenson

Comments

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Date Original

2005

Object Description

1 PDF file (127 leaves)

Language

en

File Format

application/pdf

Included in

Biology Commons

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