Dissertations and Theses @ UNI
Availability
Open Access Thesis
Keywords
Azo dyes--Toxicology; Photocatalysis; Titanium dioxide;
Abstract
C.I. Acid Orange 7 (15510) is the sodium salt of p-[(2-hydroxy-1-naphtalenyl)azo] benzenesulfonic acid. This azo dye is commonly known as Orange II and has been used to dye soaps, shoes, wood and paper (Ramchandani et al., 1994). In the United States and Europe, this dye is not allowed in foodstuffs. The purpose of this study is to investigate the efficiency of a TiO2-mediated photocatalytic oxidation process to degrade Orange II and to determine if Orange II is cytotoxic to certain types of cells. The role of UV radiation and TiO2 particles were examined, as well as the importance of oxygen in the degradation process. The effect of pH was also studied. Human fibroblasts, peripheral blood mononuclear cells (PBMCs) and Jurkat cells (lymphocyte cell line) were used to study Orange II toxicity. Saturation density and MTT (3-( 4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide dye) analysis were used to measure cell growth in the presence of increasing concentrations of Orange IL Orange II concentrations ranged from 2.5 ppm (parts per million) to 200 ppm. Similar analyses were performed on Orange II solutions before and after TiO2-mediated photocatalytic remediation. This was done to determine the efficiency of detoxification. Toxicity was also studied by using Daphnia magna.
Year of Submission
2002
Degree Name
Master of Science
Department
Department of Chemistry
First Advisor
John Bumpus
Second Advisor
Lisa Beltz
Third Advisor
Kavita Dhanwada
Date Original
2002
Object Description
1 PDF file (67 leaves)
Copyright
©2002 Arnaud Eric Nicolas Goutard
Language
en
File Format
application/pdf
Recommended Citation
Goutard, Arnaud Eric Nicolas, "Effect of TiO₂-Mediated Photocatalytic Degradation on the Toxicity of Orange II" (2002). Dissertations and Theses @ UNI. 2770.
https://scholarworks.uni.edu/etd/2770
Comments
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