Dissertations and Theses @ UNI
Availability
Open Access Thesis
Keywords
Cadherins; Chickens--Embryos; Academic theses;
Abstract
The properties of cadherin-11 adhesiveness allow it to play a dynamic role in development. Known primarily as a mesenchymal cadherin, cadherin-11 expression may be correlated with morphogenetic events in developing embryos. Although cadherin-11 expression has been studied in the mouse and Xenopus, its expression pattern in the important avian model has not been described. We investigated cadherin-11 's expression profile using a monoclonal antibody in fluorescence immunolocalization, assisted by microwave antigen retrieval; in Hamburger-Hamilton stages 13-22 chicken embryos (approx. 46-96 hrs). Cadherin-11 was expressed in mesenchymal tissue surrounding the eye, pharynx and aortic arches, auditory vesicles, lung buds and stomach, and the nasal pit. Expression in tissues of the developing peripheral nervous system included some cranial ganglia and the pineal gland rudiment. The neural tube did not express cadherin-11 except in the tail bud area undergoing secondary neurulation. Positive expression was also found in the developing circulatory system and the digestive tract. Some positive tissues included the dorsal mesocardium, the ventricle, and the pharynx, stomach and lung buds. Expression was also found in developing limb buds and in some epithelia; particularly, the epithelia that were undergoing morphogenesis. Cadherin-11 expression became more widespread as embryos developed. Our findings show that in the chick embryo, cadherin-11 is indeed a mesenchymal cadherin, but that its expression not limited to mesenchyme and could be used as a marker during organogenesis.
Year of Submission
2008
Degree Name
Master of Science
Department
Department of Biology
First Advisor
Darrell Wiens
Second Advisor
Alicia Paulson
Third Advisor
Kavita Dhanwada
Date Original
2008
Object Description
1 PDF file (64 leaves)
Copyright
©2008 Kacie Flaherty
Language
en
File Format
application/pdf
Recommended Citation
Flaherty, Kacie, "An Expression Profile of Cadherin-11 in Developing Chicken Embryos" (2008). Dissertations and Theses @ UNI. 2634.
https://scholarworks.uni.edu/etd/2634
Comments
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