Dissertations and Theses @ UNI
Availability
Open Access Thesis
Keywords
Corn--Morphology; Peptides--Separation;
Abstract
A number of similar models, i.e., Bonner's (1965) switching networks model, Kinet et al.'s (1985) evocation/determination model, Heslop-Harrison (1969) and Poethig's (1988) phytomer model, have been proposed to describe morphogenesis in plants as a product of sequential changes in cellular protein complement resulting from differential gene expression. The switch point model for Zea mays development predicts changes in the protein complements at each successive developmental stage. The objective of this study was to map, using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), the protein complement of three morphological stages of the z. mays ear: spikelet primordia (SP), spikelet primordia with glumes (SPG), and floret primordia with glumes (FPG). The 2-D PAGE gel maps for each morphological stage were compared for quantitative and/or qualitative differences in their protein complements. Of the approximately 800 plus polypeptides resolved, 23 were considered a subset associated with spikelet development. These stage-associated polypeptides showed five different patterns of occurrence coincident with spikelet morphogenesis. The majority of these polypeptides were observed at two of the three morphological stages, SP and SPG, and SP and FPG. Stage-associated polypeptides which were unique to a single stage were found for SP and SPG. No stage-associated polypeptides were unique to FPG. A distinct subset of maize ear polypeptides was discovered in the protein complements of SP, SPG, and FPG that varied in occurrence with successive organogenetic stages. This provides new evidence for the switch point model of maize inflorescence development, and supports the concepts of the switching networks model, evocation/determination model, and the phytomer model.
Year of Submission
1989
Degree Name
Master of Arts
Department
Department of Biology
First Advisor
Alan Orr
Second Advisor
Robert Seager
Third Advisor
Paul Whitson
Date Original
1989
Object Description
1 PDF file (86 leaves)
Copyright
©1989 Teresa A. Schoneman Findlay
Language
en
File Format
application/pdf
Recommended Citation
Findlay, Teresa A. Schoneman, "Polypeptides Associated With Spikelet Development in Zea mays L. Ear Organogenesis" (1989). Dissertations and Theses @ UNI. 2629.
https://scholarworks.uni.edu/etd/2629
Comments
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