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Open Access Thesis

Keywords

Ambystoma--Middle West--Genetics; Ambystoma--Conservation--Middle West; Middle West; Academic theses;

Abstract

Arguably, the primary intention of conservation genetics is to preserve allelic variability within and among populations, because a lack of genetic diversity may adversely effect the viability of a population subjected to variable environmental conditions. The influence of such evolutionary processes as genetic erosion and inbreeding depression may be pronounced for increasingly small and isolated populations. Blue-spotted salamanders (Ambystoma laterale), though widespread and relatively common throughout northeastern North America, are marked by a pair of peripherally isolated populations at the southwestern boundary of the range. These populations are putative relicts of post-glacial isolation and thus provide a model from which to ascertain the extent and rapidity of evolutionary decay in disjunct amphibian populations. Herein, the genetics of both relict populations was compared to that of a reference population, which is presumably in genetic contiguity with the greater extent of the species distribution. F-statistics, heterozygosity, effective population size, deviation from Hardy-Weinberg equilibrium expectations, and time since divergence between populations, for each population, were determined by assessing allelic variation at five polymorphic nuclear microsatellite loci. Results indicate substantial inbreeding, as well as a recent genetic bottleneck, for one of the presently isolated populations; this population, therefore, may be subject to a heightened risk of extirpation. Tangentially, the present condition of blue-spotted salamanders may be indicative of the status of other amphibian and reptilian species in the same geographic localities, given that these biota may be susceptible to many of the same environmental perturbations.

Year of Submission

2005

Degree Name

Master of Science

Department

Department of Biology

First Advisor

Theresa A. Spradling

Second Advisor

James W. Demastes

Third Advisor

Jeffrey Tamplin

Comments

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Date Original

2005

Object Description

1 PDF file (70 leaves)

Language

en

File Format

application/pdf

Included in

Biology Commons

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