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Open Access Thesis

Keywords

Ampelopsis; Leaves--Development; Academic theses;

Abstract

The genus Ampelopsis is a member of the Vitaceae. Ampelopsis cordata Michx. and Ampelopsis arborea (L.) Koehne are the only two North American species of Ampelopsis. A recent molecular phylogenetic hypothesis placed them into two different clades (Soejima and Wen, 2006). This is correlated with mature leaf morphology, i.e. A. cordata has simple leaves, and A. arborea has compound leaves. My objective was to compare the two species leaf development from initiation to mature form to determine how and at what point their developmental trajectory differentiates and to establish a method to sufficiently track leaf development. Bpi-illumination light microscopy was used to track early leaf development until just before leaf unfolding. Trajectories were identical from leafprimordium initiation to lateral leaf appendage three (LL3) formation, which initiate acropetally from the leaf primordium base. Ampelopsis arborea reiterated leaf development from initiation to LL3, for four orders of LL and two leaflet orders. Ampelopsis cordata continued LL initiation to LL5, and then second order LL initiation to LL3. Macroscopic morphometric analysis was used to track the development from unfolding to maturity. The LL and rachis and midvein branching points were tracked using the programs TPSdig (ver 1 .40), Coordgen6f, and PCAGen6n where visual growth trajectory descriptions were made. Morphometric analysis showed symmetrical development in A. arborea and asymmetrical development for A. cordata. The results from this study supported Wen' hypothesis and showed the two species in this study begin leaf development identically despite different mature forms.

Year of Submission

2008

Degree Name

Master of Science

Department

Department of Biology

First Advisor

Jean Gerrath

Second Advisor

Stephen L. O'Kane

Third Advisor

Wendy M. Olson

Comments

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Date Original

2008

Object Description

1 PDF file (76 leaves)

Language

en

File Format

application/pdf

Included in

Biology Commons

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