Dissertations and Theses @ UNI
Availability
Open Access Thesis
Keywords
Fusarium diseases of plants; Genetic polymorphisms; Academic theses;
Abstract
Fusarium head blight (FHB) of wheat and barley has reached epidemic proportions in the United States. FHB in the United States is mainly caused by Fusarium graminearum within the Fusarium species complex. Current methods such as crop rotation and the use of fungicides to control outbreaks have been poor at best. Knowledge of the organism's genome could focus and further research for new methods to control this pathogenic fungus. This study seeks to create a genetic linkage map from an intraspecific cross of the isolates Z-3939 and Z-4243, both isolated from the fields of Kansas. Amplified Fragment Length Polymorphisms (AFLP) was used to produce genetic markers and the program Map Manager QTXb20 to assemble them into a linkage map. From 44 AFLP primer pairs, 262 genetic markers were created, of these only 46 markers mapped to the 17 small linkage groups created by Map Manager QTXb20. This map was compared to a genetic linkage map created by Jurgenson et al. (2002) that used the same methods as those reported here but used progeny from an interspecific cross of isolates from Japan (R-540) and one in common with the map reported here (Z-3639). Jurgenson et al. (2002) created a marker dense map, where as the one reported here is not. This can be attributed to the difference in the type of cross used in each mapping work and population genetics analyses indicate that outcrossing is common in F graminearum.
Year of Submission
2007
Degree Name
Master of Science
Department
Department of Biology
First Advisor
James E. Jurgenson
Second Advisor
Steve L. O'Kane Jr.
Third Advisor
James Demastes
Date Original
2007
Object Description
1 PDF file (69 leaves)
Copyright
©2007 Eric Von Berns
Language
en
File Format
application/pdf
Recommended Citation
Berns, Eric Von, "The Use of AFLP to Create a Genetic Linkage Map for Fusarium graminearum" (2007). Dissertations and Theses @ UNI. 2076.
https://scholarworks.uni.edu/etd/2076
Comments
If you are the rightful copyright holder of this thesis and wish to have it removed from the Open Access Collection, please submit a request to scholarworks@uni.edu and include clear identification of the work, preferably with URL.