Archaeal Assemblages Inhabiting Temperate Mixed Forest Soil Fluctuate in Taxon Composition and Spatial Distribution over Time

Authors

Colby A. SwansonFollow
Marek K. Sliwinski, University of Northern IowaFollow

Document Type

Article

Journal/Book/Conference Title

Archaea

Volume

2013

Abstract

This study explored the persistence and spatial distribution of a diverse Archaeal assemblage inhabiting a temperate mixed forest ecosystem. Persistence under native conditions was measured from 2001 to 2010, 2011, and 2012 by comparison of 16S rRNA gene clone libraries. The Archaeal assemblages at each of these time points were found to be significantly different (AMOVA, P < 0.01), and the nature of this difference was dependent on taxonomic rank. For example, the cosmopolitan genus g_Ca. Nitrososphaera (I.1b) was detected at all time points, but within this taxon the abundance of s_SCA1145, s_SCA1170, and s_Ca. N. gargensis fluctuated over time. In addition, spatial heterogeneity (patchiness) was measured at these time points using 1D TRFLP-SSCP fingerprinting to screen soil samples covering multiple spatial scales. This included soil collected from small volumes of 3 cubic centimeters to larger scales—over a surface area of 50 m², plots located 1.3 km apart, and a separate locality 23 km away. The spatial distribution of Archaea in these samples changed over time, and while g_Ca. Nitrososphaera (I.1b) was dominant over larger scales, patches were found at smaller scales that were dominated by other taxa. This study measured the degree of change for Archaeal taxon composition and patchiness over time in temperate mixed forest soil.

Department

Department of Biology

Comments

First published in Archaea, v. 2013, Article ID 870825, published by Hindawi Publishing Corporation. DOI: 10.1155/2013/870825

Original Publication Date

2013

DOI of published version

10.1155/2013/870825

Repository

UNI ScholarWorks, University of Northern Iowa, Rod Library

Date Digital

2013

Copyright

©2013 Colby A. Swanson and Marek K. Sliwinski. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Language

EN

File Format

application/pdf

Recommended Citation

Swanson, Colby A. and Sliwinski, Marek K., "Archaeal Assemblages Inhabiting Temperate Mixed Forest Soil Fluctuate in Taxon Composition and Spatial Distribution over Time" (2013). Faculty Publications. 10.
https://scholarworks.uni.edu/bio_facpub/10