2022 Summer Undergraduate Research Program (SURP) Symposium

Location

ScholarSpace, Rod Library, University of Northern Iowa

Presentation Type

Open Access Poster Presentation

Document Type

poster

Abstract

Recombinase proteins, such as RAD51, play a central role in repairing double-strand breaks of DNA through the process of homologous recombination. In this project, Atomic Force Microscopy (AFM) was used to generate scans of RAD51 proteins bound to double-stranded DNA. Due to the cooperativity of RAD51 binding, we observed a nucleation effect where RAD51 proteins generally tended to bind to DNA that already had other RAD51 proteins bound.

Start Date

29-7-2022 11:00 AM

End Date

29-7-2022 1:30 PM

Event Host

Summer Undergraduate Research Program, University of Northern Iowa

Faculty Advisor

Justin Peters

Department

Department of Chemistry and Biochemistry

File Format

application/pdf

Available for download on Monday, July 29, 2024

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Jul 29th, 11:00 AM Jul 29th, 1:30 PM

Using Atomic Force Microscopy to Study DNA and RAD51 Protein Interaction

ScholarSpace, Rod Library, University of Northern Iowa

Recombinase proteins, such as RAD51, play a central role in repairing double-strand breaks of DNA through the process of homologous recombination. In this project, Atomic Force Microscopy (AFM) was used to generate scans of RAD51 proteins bound to double-stranded DNA. Due to the cooperativity of RAD51 binding, we observed a nucleation effect where RAD51 proteins generally tended to bind to DNA that already had other RAD51 proteins bound.