2022 Summer Undergraduate Research Program (SURP) Symposium
Location
ScholarSpace, Rod Library, University of Northern Iowa
Presentation Type
Open Access Poster Presentation
Document Type
poster
Keywords
Genetic recombination;
Abstract
Recombinase proteins, such as RAD51, play a central role in repairing double-strand breaks of DNA through the process of homologous recombination. In this project, Atomic Force Microscopy (AFM) was used to generate scans of RAD51 proteins bound to double-stranded DNA. Due to the cooperativity of RAD51 binding, we observed a nucleation effect where RAD51 proteins generally tended to bind to DNA that already had other RAD51 proteins bound.
Start Date
29-7-2022 11:00 AM
End Date
29-7-2022 1:30 PM
Event Host
Summer Undergraduate Research Program, University of Northern Iowa
Faculty Advisor
Justin Peters
Department
Department of Chemistry and Biochemistry
Copyright
©2022 Samrin Shahnaz and Dr. Justin Peters
File Format
application/pdf
Recommended Citation
Shahnaz, Samrin and Peters, Justin Ph.D., "Using Atomic Force Microscopy to Study DNA and RAD51 Protein Interaction" (2022). Summer Undergraduate Research Program (SURP) Symposium. 19.
https://scholarworks.uni.edu/surp/2022/all/19
Using Atomic Force Microscopy to Study DNA and RAD51 Protein Interaction
ScholarSpace, Rod Library, University of Northern Iowa
Recombinase proteins, such as RAD51, play a central role in repairing double-strand breaks of DNA through the process of homologous recombination. In this project, Atomic Force Microscopy (AFM) was used to generate scans of RAD51 proteins bound to double-stranded DNA. Due to the cooperativity of RAD51 binding, we observed a nucleation effect where RAD51 proteins generally tended to bind to DNA that already had other RAD51 proteins bound.
