2021 Summer Undergraduate Research Program (SURP) Symposium
Location
Ballroom, Maucker Student Union, University of Northern Iowa
Presentation Type
Poster Presentation (UNI Access Only)
Document Type
poster
Keywords
DNA repair; Recombinant DNA;
Abstract
A crucial step in the DNA repair mechanism of homologous recombination is the search for homology between DNA molecules which is closely followed by strand exchange between homologous DNA. This step is governed by the nucleoprotein RAD51 which competes with Replication Protein A (RPA) to saturate single-stranded DNA (ssDNA) overhangs. Such interactions are naturally stochastic, so a dynamic stochastic model is the best representation of the real molecular process occurring during homologous recombination. Forming a stochastic Monte Carlo model of the competition between RAD51 and RPA allows for the observation of real-time dynamics during these interactions. Such a dynamic model was developed in Matlab and is being used to study the effects that various governing parameters have on each protein’s saturation level on ssDNA.
Start Date
30-7-2021 11:30 AM
End Date
30-7-2021 1:15 PM
Event Host
Summer Undergraduate Research Program, University of Northern Iowa
Faculty Advisor
Ali Tabei
Department
Department of Physics
Copyright
©2021 Aaron Kirchman and Ali Tabei
File Format
application/pdf
Recommended Citation
Kirchman, Aaron and Tabei, Ali Ph.D., "Stochastic Monte Carlo Simulations of Competing Nucleoproteins on Single-Stranded DNA" (2021). Summer Undergraduate Research Program (SURP) Symposium. 29.
https://scholarworks.uni.edu/surp/2021/all/29
Stochastic Monte Carlo Simulations of Competing Nucleoproteins on Single-Stranded DNA
Ballroom, Maucker Student Union, University of Northern Iowa
A crucial step in the DNA repair mechanism of homologous recombination is the search for homology between DNA molecules which is closely followed by strand exchange between homologous DNA. This step is governed by the nucleoprotein RAD51 which competes with Replication Protein A (RPA) to saturate single-stranded DNA (ssDNA) overhangs. Such interactions are naturally stochastic, so a dynamic stochastic model is the best representation of the real molecular process occurring during homologous recombination. Forming a stochastic Monte Carlo model of the competition between RAD51 and RPA allows for the observation of real-time dynamics during these interactions. Such a dynamic model was developed in Matlab and is being used to study the effects that various governing parameters have on each protein’s saturation level on ssDNA.
