•  
  •  
 

Document Type

Research

Keywords

gnotobiology, axenic culture, sterile culture, germ-free culture

Abstract

An economical plant growth chamber, 16 cm in diameter and 50 cm high, was made from two 4-liter glass beakers. The bottom was removed from one beaker, and a 1 cm hole cut in the bottom of the other. The bottomless beaker, when placed atop the other, telescoped slightly for firm support. Sand was placed in the bottom of the chamber as a growth substrate. A polyethylene bag was placed over the two beakers and filtered air (under pressure) and nutrient solution entered the chamber through rubber tubing. Six chambers, each with an open petri dish of nutrient agar in the bottom, were tested for 2 weeks, and all dishes remained microbe-free. The laminar flow of air between the plastic bag and the glass walls of the beakers prevented microbes from entering the chamber. The chambers supported good growth of corn and soybean seedlings for 4 weeks when a sterile nutrient solution was added to the sand as needed.

Publication Date

June 1986

Journal Title

Proceedings of the Iowa Academy of Science

Volume

93

Issue

2

First Page

51

Last Page

53

Copyright

© Copyright 1986 by the Iowa Academy of Science, Inc.

Language

EN

File Format

application/pdf

Share

COinS
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.