The Use of 2,4-Dinitrophenylhydrazine Derivatives of Ovarian Ketosteroids in Steroid Analysis

Authors

Asa C. Black Jr., University of IowaFollow
G. Rodman Davenport, Vanderbilt University
James R. West, University of Iowa

Document Type

Research

Keywords

ovarian Ketosteroids, 2, 4-Dinitrophenylhydrazine derivatives, steroid analysis

Abstract

This paper reports the development of a technically simple analytical technique for the characterization and quantitative determination of ovarian ketosteroids employing derivative formation with 2,4-dinitrophenylhydrazine (2,4-DNP), separation by thin-layer chromatography (TLC), and quantitative determination by spectrophotometry. The chemical identities of the derivatives were established by nitrogen analysis. The melting point, chromatographic mobilities of the free steroids and their derivatives on silica gel G in three systems, wavelength of maximum absorption in chloroform and ethanol, and molar absorption coefficient are reported for the 2,4-DNP derivatives of ovarian ketosteroids. The relationship between the absorption maximum in chloroform and the chemical structure of the derivatives has been analyzed.

Publication Date

September 1978

Journal Title

Proceedings of the Iowa Academy of Science

Volume

85

Issue

3

First Page

99

Last Page

102

Copyright

©1978 Iowa Academy of Science, Inc.

Language

en

File Format

application/pdf

Recommended Citation

Black, Asa C. Jr.; Davenport, G. Rodman; and West, James R. (1978) "The Use of 2,4-Dinitrophenylhydrazine Derivatives of Ovarian Ketosteroids in Steroid Analysis," Proceedings of the Iowa Academy of Science, 85(3), 99-102.
Available at: https://scholarworks.uni.edu/pias/vol85/iss3/8