Home > Iowa Academy of Science > Journals & Newsletters > Proceedings of the Iowa Academy of Science > Volume 83 (1976) > Number 4
Document Type
Research
Abstract
Protuberances tentatively identified as buds were initiated on callus proliferating from the bases of excised terminal buds of Psychotria punctata (Rubiaceae) cultured on media containing 10(-4) and 10(-5)M zeatin. Only those cultures maintained in a greenhouse during mid-summer formed such buds. A high cytokinin concentration together with one or more specific environmental conditions appear to be required for their formation. In contrast, large nodule-bearing shoots developed directly from terminal buds when these were excised during the fall and cultured on the same medium in controlled environment chambers providing temperatures and light intensities lower than in the greenhouse. Zeatin was required for shoot development on this medium; little development occurred in its absence. Shoot development occurred in both 8- and 16-hr days in the chambers; it did not occur in darkness, in continuous, predominantly incandescent light, or in the greenhouse.
Publication Date
December 1976
Journal Title
Proceedings of the Iowa Academy of Science
Volume
83
Issue
4
First Page
130
Last Page
132
Copyright
©1976 Iowa Academy of Science, Inc.
Language
en
File Format
application/pdf
Recommended Citation
Edwards, Wayne J. and LaMotte, Clifford E.
(1976)
"Bud Formation and Shoot Development In Vitro: Observations on Stem and Bud Explants of Psychotria punctata (Rubiaceae),"
Proceedings of the Iowa Academy of Science, 83(4), 130-132.
Available at:
https://scholarworks.uni.edu/pias/vol83/iss4/5