Nitrogen Metabolism in Acanthamoeba castellanii: Amino Acid Consumption and Composition Patterns

Authors

Warren D. Dolphin, Iowa State University

Document Type

Research

Keywords

Amino Acids, Nutrition, Acanthamoeba

Abstract

Quantitative analyses of the amino acids in the culture media before and after population growth demonstrate that Acanthamoeba castellanii selectively utilizes amino acids from Adam's defined medium (DM₂) and Neff's optimal growth medium, but no amino acid is completely depleted during normal population growth. The cellular amino acid content varies for amoebae grown in the two media. The most common total cellular amino acids are alanine, aspartic acid, glutamic acid, glycine, and leucine. The most common free amino acids in the intracellular pools are alanine and proline. In DM₂, NH₃ is produced at a rate of 0.027 picomoles/ amoebae/hour.

Publication Date

May 1976

Journal Title

Proceedings of the Iowa Academy of Science

Volume

82

Issue

3-4

First Page

171

Last Page

174

Copyright

©1976 Iowa Academy of Science, Inc.

Language

en

File Format

application/pdf

Recommended Citation

Dolphin, Warren D. (1976) "Nitrogen Metabolism in Acanthamoeba castellanii: Amino Acid Consumption and Composition Patterns," Proceedings of the Iowa Academy of Science, 82(3-4), 171-174.
Available at: https://scholarworks.uni.edu/pias/vol82/iss3/9