A Combination of Chromic Acid, Acetic Acid and Formalin as a Fixative for Animal Tissues

Authors

H. W. NorrisFollow

Document Type

Research

Abstract

That many of the fixing reagents in common use in the preparation of tissues for histological study are poorly adapted to the fixation of the ova and embryos of Amphibians, is evident to anyone who gives more than casual attention to the matter. The large amount of yolk present in the cells of the Amphibian embryo makes completely uniform fixation with some reagents impossible, and then, too, the disintegrating effect of many fixing fluids upon the yoke granules makes the result very unsatisfactory. A fluid that gives good fixation is likely to interfere with subsequent staining, as witness most of the chromic acid and osmic acid compounds.

Publication Date

1900

Journal Title

Proceedings of the Iowa Academy of Sciences

Volume

8

Issue

1

First Page

78

Last Page

80

Copyright

©1900 Iowa Academy of Science, Inc.

Language

en

File Format

application/pdf

Recommended Citation

Norris, H. W. (1900) "A Combination of Chromic Acid, Acetic Acid and Formalin as a Fixative for Animal Tissues," Proceedings of the Iowa Academy of Science, 8(1), 78-80.
Available at: https://scholarworks.uni.edu/pias/vol8/iss1/10