The growth and morphology of a caecal porcine trichomonad (probably Tritrichomonas rotunda) were studied from both the trichomonad's natural environment, the pig's caecum, and in culture. Growth of the organism was studied in C.P.L.M. medium at 37° C. After an initial inoculum of 100 organisms per ml. the population rose to 2,900,000 per ml. on day 4 and then decreased to near extinction on day 7. In comparison to the organisms on stained smears made directly from the pig's caecum, the organisms after extensive subculturing (100 subcultures over a period of 20 months) were more elongate and often almost tubular; possessed longer undulating membranes and costas approaching full body length instead of only ½ -1/3 body length; had axostyles with a more bulbous capitulum; and had club-shaped parabasal bodies instead of V-shaped structures. No significant variations were found in the morphology of the trichomonads during the various days of the culture cycle. Because of these morphological changes during extended subculturing, the interpretation of the morphological structures based upon trichomonads after extended periods of subculturing must be viewed with caution.
Proceedings of the Iowa Academy of Science
©1968 Iowa Academy of Science, Inc.
Buttrey, Benton W.
"Studies on Growth and Morphology of a Trichomonad from the Caecum of the Pig,"
Proceedings of the Iowa Academy of Science, 75(1), 390-396.
Available at: https://scholarworks.uni.edu/pias/vol75/iss1/52