Anthocyanin Pigments in the Genus Lobelia: I. Pigments in Lobelia Cardinalis L. and L. Siphilitica L.

Authors

S. Lane Wilson, Drake UniversityFollow

Document Type

Research

Abstract

The corollas of L. cardinalis and L. siphilitica were extracted in 1 percent HCl in methanol for 24 hours. This extract (10 ml. portions) was streaked on 3 mm. Whatman filterpaper. The filterpaper was placed in a large chromatographic tank in a solvent system of n-butanol: acetic acid: water (4:1:5 v/v). After separation the bands were cut out and the pigment from each band eluted in a solution of 0.01 percent HCI in methanol. These solutions were then reduced in volume under vacuum and the absorption spectrum of each pigment determined. Aliquots of the crude extract was also spotted on Whatman No. 1 filterpaper and the Rf values of the pigments determined in four different solvent systems. From these data, the main pigment found in L. cardinalis is pelargonidin-3-monoglucoside, and the main pigment in L. siphilitica is a delphinidin-3,5-diglucoside.

Publication Date

1968

Journal Title

Proceedings of the Iowa Academy of Science

Volume

75

Issue

1

First Page

65

Last Page

68

Copyright

©1968 Iowa Academy of Science, Inc.

Language

en

File Format

application/pdf

Recommended Citation

Wilson, S. Lane (1968) "Anthocyanin Pigments in the Genus Lobelia: I. Pigments in Lobelia Cardinalis L. and L. Siphilitica L.," Proceedings of the Iowa Academy of Science, 75(1), 65-68.
Available at: https://scholarworks.uni.edu/pias/vol75/iss1/13