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Document Type

Research

Abstract

Two structurally similar cardiac glycosides, digoxin and digitoxin, and their aglycones, digoxigenin and digitoxigenin, were separated by thin-layer chromatography using glass plates coated with silica gel H. Of 50 solvent systems tried, 15 were found to effect excellent separation of digoxin and digoxigenin. Separation of digoxin and digitoxin and their respective aglycones was achieved with three solvent systems. Spots were visualized with meta-dinitrobenzene sodium hydroxide sprays. The lower limit of detection was 10 micrograms of glycoside per square centimeter. This method of separation should prove significant in identifying drug metabolites and determining toxicity levels of cardiac glycosides.

Publication Date

1966

Journal Title

Proceedings of the Iowa Academy of Science

Volume

73

Issue

1

First Page

101

Last Page

106

Copyright

© Copyright 1966 by the Iowa Academy of Science, Inc.

Language

EN

File Format

application/pdf

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