Two structurally similar cardiac glycosides, digoxin and digitoxin, and their aglycones, digoxigenin and digitoxigenin, were separated by thin-layer chromatography using glass plates coated with silica gel H. Of 50 solvent systems tried, 15 were found to effect excellent separation of digoxin and digoxigenin. Separation of digoxin and digitoxin and their respective aglycones was achieved with three solvent systems. Spots were visualized with meta-dinitrobenzene sodium hydroxide sprays. The lower limit of detection was 10 micrograms of glycoside per square centimeter. This method of separation should prove significant in identifying drug metabolites and determining toxicity levels of cardiac glycosides.
Proceedings of the Iowa Academy of Science
©1966 Iowa Academy of Science, Inc.
White, B. J. and Oeth, Dennis
"Separation of Cardiac Glycosides by Thin-Layer Chromatography,"
Proceedings of the Iowa Academy of Science, 73(1), 101-106.
Available at: https://scholarworks.uni.edu/pias/vol73/iss1/17