Home > Iowa Academy of Science > Journals & Newsletters > Proceedings of the Iowa Academy of Science > Volume 73 (1966) > Annual Issue
Document Type
Research
Abstract
Two structurally similar cardiac glycosides, digoxin and digitoxin, and their aglycones, digoxigenin and digitoxigenin, were separated by thin-layer chromatography using glass plates coated with silica gel H. Of 50 solvent systems tried, 15 were found to effect excellent separation of digoxin and digoxigenin. Separation of digoxin and digitoxin and their respective aglycones was achieved with three solvent systems. Spots were visualized with meta-dinitrobenzene sodium hydroxide sprays. The lower limit of detection was 10 micrograms of glycoside per square centimeter. This method of separation should prove significant in identifying drug metabolites and determining toxicity levels of cardiac glycosides.
Publication Date
1966
Journal Title
Proceedings of the Iowa Academy of Science
Volume
73
Issue
1
First Page
101
Last Page
106
Copyright
©1966 Iowa Academy of Science, Inc.
Language
en
File Format
application/pdf
Recommended Citation
White, B. J. and Oeth, Dennis
(1966)
"Separation of Cardiac Glycosides by Thin-Layer Chromatography,"
Proceedings of the Iowa Academy of Science, 73(1), 101-106.
Available at:
https://scholarworks.uni.edu/pias/vol73/iss1/17