Phenol:water and butanol:acetic acid:water solvent systems were used with horizontal and descending paper chromatography of the body mucuses of Carpiodes, Catostomus, Ictiobus, Moxostoma, and Hypentelium. Mucus could be sampled in the field, applied to the chromatography paper, allowed to dry, and kept for several days without refrigeration. Chromatograms of fresh and dried mucus appeared the same. Horizontal runs were faster but were abandoned for the greater separation possible with descending techniques. Ninhydrin-stained descending chromatograms showed differences between some genera within a run. Descending chromatograms run in butanol:acetic acid:water and viewed with short wave ultraviolet light showed differences between most genera studied. The pattern seen depended on the mucus and the intensity and the wavelength of the ultraviolet light. There seemed to be no effect of age, sex, or area of collection of the fishes on the pattern. Chromatograms of the mucuses of Catostomus, Hypentelium, and Moxostoma, members of the subfamily Catostominae, all showed prominent fluorescent spots under ultraviolet light, while the chromatograms of the Carpiodes species studied (subfamily Ictiobinae) lacked this fluorescence.
Proceedings of the Iowa Academy of Science
©1964 Iowa Academy of Science, Inc.
Huntsman, Gene R.
"Paper Chromatograms of Body Mucus of Some Suckers (Family Catostomidae),"
Proceedings of the Iowa Academy of Science, 71(1), 263-274.
Available at: https://scholarworks.uni.edu/pias/vol71/iss1/43