Home > Iowa Academy of Science > Journals & Newsletters > Proceedings of the Iowa Academy of Science > Volume 70 (1963) > Annual Issue
Document Type
Research
Abstract
Wittman's aceto-iron-haematoxylin stain was combined with Hoyer's mounting medium in a rapid method for demonstrating mitosis in the green algae. Both filamentous and unicellular forms were killed, fixed, and stained in one step with the self-mordanting stain. Cells were then washed with acetic acid and transferred directly to the water-soluble Hoyer's medium. Slides prepared in this manner are permanent. Chlorophyll was bleached from the algal cells by this medium. This revealed the stained nuclei and mitotic figures. This simplified technique is particularly suitable for student use.
Publication Date
1963
Journal Title
Proceedings of the Iowa Academy of Science
Volume
70
Issue
1
First Page
138
Last Page
142
Copyright
©1963 Iowa Academy of Science, Inc.
Language
en
File Format
application/pdf
Recommended Citation
Bowen, William R.
(1963)
"The Demonstration of Mitotic Figures in Green Algae,"
Proceedings of the Iowa Academy of Science, 70(1), 138-142.
Available at:
https://scholarworks.uni.edu/pias/vol70/iss1/29