Manometric studies were employed to assay for ascorbic acid oxidase activity in cell free homogenates from the mycelium of the mold Myrothecium verrucaria. It was found that gas exchange measurements deviated from expected stoichiometry based on the utilization of one-half of a mole of oxygen for every mole of ascorbate oxidized. Oxygen was consumed in excess of the expected amount and carbon dioxide was produced during the course of the reaction. Apparently, the ascorbic acid oxidase reaction which produces dehydroascorbic acid is accompanied by or followed by other reactions involving oxygen consumption and carbon dioxide evolution.
Proceedings of the Iowa Academy of Science
©1962 Iowa Academy of Science, Inc.
Lillehoj, E. B. and Smith, F. G.
"Anomalous Gas Exchange in Ascorbate Oxidation by the Myrothecium Enzyme,"
Proceedings of the Iowa Academy of Science, 69(1), 193-199.
Available at: https://scholarworks.uni.edu/pias/vol69/iss1/32