Celestine Blue B as a Nuclear Stain in Vaginal Exfoliative Cytology

Authors

A. R. Dzmura, College of Osteopathic Medicine and SurgeryFollow
S. D. Miroyiannis, College of Osteopathic Medicine and SurgeryFollow
E. A. Burrows, College of Osteopathic Medicine and Surgery

Document Type

Research

Abstract

This paper deals with spectrophotometric and chromatographic analyses of the dye and a study of two staining solutions with a variety of counterstains, using material fixed with ether/ alcohol. These observations indicate that the optimal combination of solutions are 0.5 gm celestine blue B and 40 ml glacial acetic acid heated to 60° C with stirring, to which when completely dissolved 100 ml water and 140 ml 4% ferric alum are slowly added. This should be incorporated with the Papanicolau procedure with the sole omissions of hematoxylin, the acid bath, and the lithium carbonate bath. The result is a very acceptable slide with all the advantages of the Papanicolau technique while employing a nuclear stain that is not hematoxylin, but which has high nuclear specificity. Slides have remained light-fast for at least one year.

Publication Date

1961

Journal Title

Proceedings of the Iowa Academy of Science

Volume

68

Issue

1

First Page

586

Last Page

593

Copyright

©1961 Iowa Academy of Science, Inc.

Language

en

File Format

application/pdf

Recommended Citation

Dzmura, A. R.; Miroyiannis, S. D.; and Burrows, E. A. (1961) "Celestine Blue B as a Nuclear Stain in Vaginal Exfoliative Cytology," Proceedings of the Iowa Academy of Science, 68(1), 586-593.
Available at: https://scholarworks.uni.edu/pias/vol68/iss1/82