A Modification of the Carbowax Embedding Technique for Plant Tissue

Authors

Waldo S. WalkerFollow

Document Type

Research

Abstract

The highly hydrate condition of the wall material of the cells of collenchyma causes considerable difficulty in the preparation of permanent sections of the material which are suitable for quantitative studies. When standard procedures of dehydration and embedding are employed, excessive shrinkage of the wall material is common. In an attempt to avoid this shrinkage, polyethylene glycol (carbowax) was used as the killing, fixing, dehydrating and embedding medium. This method met with considerable success. The schedule outlined is a modification of that used by A. R. Spurr.

Publication Date

1959

Journal Title

Proceedings of the Iowa Academy of Science

Volume

66

Issue

1

First Page

81

Last Page

85

Copyright

©1959 Iowa Academy of Science, Inc.

Language

en

File Format

application/pdf

Recommended Citation

Walker, Waldo S. (1959) "A Modification of the Carbowax Embedding Technique for Plant Tissue," Proceedings of the Iowa Academy of Science, 66(1), 81-85.
Available at: https://scholarworks.uni.edu/pias/vol66/iss1/9