Certain Mitotic Effects of Kinetin, Gibberellic Acid, Maleic Hydrazide, and Indoleacetic Acid in Onion Roots

Authors

Mary Annunciata McManusFollow

Document Type

Research

Abstract

Growing, intact, adventitious roots from the bulbs of Allium cepa were treated with 1 ppm and 10 ppm solutions of kinetin, gibberellic acid, maleic hydrazide, and indoleacetic acid, and with paired combinations of these. The effect on the rate of mitosis was studied by counting the mitotic figures present in the distal 5 mm. of the root tip in serial longitudinal sections. Roots treated with kinetin alone or in any combination showed marked inhibition of mitosis. There was initial inhibition followed by an after-effect of stimulation of mitosis in roots treated with gibberellic acid, with indoleacetic acid, or with a combination of these. Maleic hydrazide did not reverse the action of any of the auxin-like substances tested, but the after-effect of stimulation was not seen when it was present. Roots treated with maleic hydrazide contained many cells in which the chromosomes showed aberrations including fragmentation and changes which gave the chromosomes a beaded appearance.

Publication Date

1959

Journal Title

Proceedings of the Iowa Academy of Science

Volume

66

Issue

1

First Page

74

Last Page

80

Copyright

©1959 Iowa Academy of Science, Inc.

Language

en

File Format

application/pdf

Recommended Citation

McManus, Mary Annunciata (1959) "Certain Mitotic Effects of Kinetin, Gibberellic Acid, Maleic Hydrazide, and Indoleacetic Acid in Onion Roots," Proceedings of the Iowa Academy of Science, 66(1), 74-80.
Available at: https://scholarworks.uni.edu/pias/vol66/iss1/8