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Document Type

Research

Abstract

The technique of paper electrophoresis was employed to develop a method for the separation of the end-products of salicylate metabolism. A phthalate buffer with a pH of 3.2 and an ionic strength of 0.0125 to 0.05 produced the most satisfactory separation of the metabolites. It was found that the end-products, i.e., salicylic, acetyl salicylic, salicyluric, and gentisic acids, could best be detected by the fluorescent spots they exhibited when the paper strips were viewed under ultraviolet light. The sensitivity of the method was such that as little as one microgram of salicylic acid could be detected as a fluorescent spot after electrophoretic separation.

Publication Date

1959

Journal Title

Proceedings of the Iowa Academy of Science

Volume

66

Issue

1

First Page

205

Last Page

209

Copyright

© Copyright 1959 by the Iowa Academy of Science, Inc.

Language

EN

File Format

application/pdf

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