The Separation of Salicylate Metabolites By Paper Electrophoresis

Authors

Janice Franz, State University of Iowa
Dorothy Schottelius, State University of Iowa
Emilio Arredondo, State University of IowaFollow
W. D. Paul, State University of IowaFollow
J. I. Routh, State University of IowaFollow

Document Type

Research

Abstract

The technique of paper electrophoresis was employed to develop a method for the separation of the end-products of salicylate metabolism. A phthalate buffer with a pH of 3.2 and an ionic strength of 0.0125 to 0.05 produced the most satisfactory separation of the metabolites. It was found that the end-products, i.e., salicylic, acetyl salicylic, salicyluric, and gentisic acids, could best be detected by the fluorescent spots they exhibited when the paper strips were viewed under ultraviolet light. The sensitivity of the method was such that as little as one microgram of salicylic acid could be detected as a fluorescent spot after electrophoretic separation.

Publication Date

1959

Journal Title

Proceedings of the Iowa Academy of Science

Volume

66

Issue

1

First Page

205

Last Page

209

Copyright

©1959 Iowa Academy of Science, Inc.

Language

en

File Format

application/pdf

Recommended Citation

Franz, Janice; Schottelius, Dorothy; Arredondo, Emilio; Paul, W. D.; and Routh, J. I. (1959) "The Separation of Salicylate Metabolites By Paper Electrophoresis," Proceedings of the Iowa Academy of Science, 66(1), 205-209.
Available at: https://scholarworks.uni.edu/pias/vol66/iss1/30