A Comparison of Plasma Protein Fractions by Kjeldahl and Biuret Analysis and Refractive Index Measurements

Authors

R. William Knouse, State University of IowaFollow
J. I. Routh, State University of IowaFollow

Document Type

Research

Abstract

In electrophoretic studies of serum and plasma the concentration of protein in the various fractions is often expressed in absolute values. These are commonly obtained by calculation from the percentage distribution of the components and the total protein content of the serum or plasma. Although this practice is widespread there is no assurance that the values obtained reflect a true measure of the protein content of the individual components. It is readily apparent from the divergent results obtained by the technique of paper electrophoresis that the major components of serum contain varying concentrations of protein, lipids and carbohydrates and exhibit a varying response to protein dyes.

Publication Date

1958

Journal Title

Proceedings of the Iowa Academy of Science

Volume

65

Issue

1

First Page

241

Last Page

245

Copyright

©1958 Iowa Academy of Science, Inc.

Language

en

File Format

application/pdf

Recommended Citation

Knouse, R. William and Routh, J. I. (1958) "A Comparison of Plasma Protein Fractions by Kjeldahl and Biuret Analysis and Refractive Index Measurements," Proceedings of the Iowa Academy of Science, 65(1), 241-245.
Available at: https://scholarworks.uni.edu/pias/vol65/iss1/34