"Forssman hapten" is a term applied to a complex antigenic substance which, when injected into rabbits, elicits the production of a serum antibody that is capable of hemolyzing sheep erythrocytes in the presence of complement. The organs of the guinea pig served as the source of the antigen in Forssman's original experiments, but it is known to occur in a considerable number of other living organisms. The kidney of the horse is a particularly good source of true forssman hapten (Brunius, 1936). It has been shown that alcoholic extract of horse kidney, made according to the method which Brunius found satisfactory for obtaining the source material of the Forssman hapten, is capable of potentiating agglutination of duck erythrocytes by chicken plasma (Becker and Schwink, 1953). The destruction of duck erythrocytes labeled with Plasmodium lophurae in the blood stream of chicks, however, was not significantly accelerated by intravenous injections of the same horse kidney extract. Though the reason for the negative outcome was not known, one possibility was considered to be stronger affinity of the fixed tissues for the extract than of the blood plasma. Since the coccidium Eimeria tenella undergoes the greater part of its asexual and sexual development in the mucosa of the caeca of chickens, tests were made of the effect of injections of horse kidney extract on the development of this parasite.
Proceedings of the Iowa Academy of Science
©1953 Iowa Academy of Science, Inc.
Becker, Elery R. and Zimmerman, William J.
"Influence of Alcoholic Extract of Horse Kidney on Eimeria tenella Infection in Chicks,"
Proceedings of the Iowa Academy of Science, 60(1), 574-578.
Available at: https://scholarworks.uni.edu/pias/vol60/iss1/80