Home > Iowa Academy of Science > Journals & Newsletters > Proceedings of the Iowa Academy of Science > Volume 48 (1941) > Annual Issue
Document Type
Research
Abstract
In the technique reported by Stiles and Eastwood (Iowa Acad. Sci. Proc. 1940) difficulty was encountered in floating Diglycol Stearate sections upon glass slides. The usual water albumen method of affixing ribbons upon slides could not be used satisfactorily for two reasons: first, because Diglycol is soluble in water, thus causing the ribbon to disintegrate before the tissue is permanently affixed to the slide and secondly because the surface tension of water causes a spreading effect of the ribbon. The difficulties of affixing the tissue to the slide when it is Diglycol embedded constituted a serious weakness in this technique. This investigation was undertaken in an attempt to find a floating agent which would not dissolve Diglycol ribbons in any appreciable amount. The use of calcium chloride as a floating agent was suggested by C. E. Moritz. Its solubility was tested in the usual manner, and it was found that Diglycol was not dissolved by it.
Publication Date
1941
Journal Title
Proceedings of the Iowa Academy of Science
Volume
48
Issue
1
First Page
484
Last Page
484
Copyright
©1941 Iowa Academy of Science, Inc.
Language
en
File Format
application/pdf
Recommended Citation
Stiles, Karl A. and Heck, Kenneth
(1941)
"A Method of Mounting Diglycol Stearate Sections upon Slides (Abstract),"
Proceedings of the Iowa Academy of Science, 48(1), 484-484.
Available at:
https://scholarworks.uni.edu/pias/vol48/iss1/126