One of the problems commonly encountered in the preparation of tissue for histological studies is the tendency for it to become excessively hardened before sectioning with a rotary microtome. The purpose of the investigation was to discover by as objective a means as possible not only the hardening effects of various agents on tissues, but also the influence of the time element. The approach to this problem has been to test the hardness of the tissue with a standard penetration needle after each step in the various techniques used.
Proceedings of the Iowa Academy of Science
©1941 Iowa Academy of Science, Inc.
Stiles, Karl A. and Stewart, Thomas N.
"A Comparative Study of the Hardening Effects of Various Fixatives, Dehydrating, Clearing, and Embedding Agents,"
Proceedings of the Iowa Academy of Science, 48(1), 423-424.
Available at: https://scholarworks.uni.edu/pias/vol48/iss1/114