Differences in the Phosphatase Systems of Plant and Animal Tissue by a Michrotechnic

Authors

James J. Hilbe, Dowling CollegeFollow
T. U. Marron, Iowa Lutheran HospitalFollow

Document Type

Research

Abstract

Gomori¹ has shown the location of phosphatase m animal organs by a michrotechnic. The principle is as follows: tissue sections are incubated in the presence of a substrate. Phosphate ions will be split off by the enzyme and in the presence of the calcium ion will form insoluble calcium phosphate at the point of liberation. Calcium phosphate can be converted to a dark colored insoluble precipitate. The visible precipitate then indicates the location of the phosphatase. The purpose of this research has been to subject the plant material to a similar procedure and so demonstrate any similarity or dissimilarity to what is known concerning animal material.

Publication Date

1940

Journal Title

Proceedings of the Iowa Academy of Science

Volume

47

Issue

1

First Page

235

Last Page

237

Copyright

©1940 Iowa Academy of Science, Inc.

Language

en

File Format

application/pdf

Recommended Citation

Hilbe, James J. and Marron, T. U. (1940) "Differences in the Phosphatase Systems of Plant and Animal Tissue by a Michrotechnic," Proceedings of the Iowa Academy of Science, 47(1), 235-237.
Available at: https://scholarworks.uni.edu/pias/vol47/iss1/45