Gomori1 has shown the location of phosphatase m animal organs by a michrotechnic. The principle is as follows: tissue sections are incubated in the presence of a substrate. Phosphate ions will be split off by the enzyme and in the presence of the calcium ion will form insoluble calcium phosphate at the point of liberation. Calcium phosphate can be converted to a dark colored insoluble precipitate. The visible precipitate then indicates the location of the phosphatase. The purpose of this research has been to subject the plant material to a similar procedure and so demonstrate any similarity or dissimilarity to what is known concerning animal material.
Proceedings of the Iowa Academy of Science
©1940 Iowa Academy of Science, Inc.
Hilbe, James J. and Marron, T. U.
"Differences in the Phosphatase Systems of Plant and Animal Tissue by a Michrotechnic,"
Proceedings of the Iowa Academy of Science, 47(1), 235-237.
Available at: https://scholarworks.uni.edu/pias/vol47/iss1/45