Previous work of Magee and Smith has shown that the determination of reducing substances in beef-broth media by the Shaffer-Hartman micro method, following clarification with lead sub-acetate, gives results that are extremely variable. It was found impossible to completely recover known quantities of glucose added to the medium.
Proceedings of the Iowa Academy of Science
©1932 Iowa Academy of Science, Inc.
McCreary, E. J. and Smith, H. G.
"A Study of Methods for the Determination of Reducing Sugar in Bacteriological Media II,"
Proceedings of the Iowa Academy of Science, 39(1), 171-173.
Available at: https://scholarworks.uni.edu/pias/vol39/iss1/35