The Micro Determination of Citric Acid by the Thunberg Methylene Blue Method

Authors

Adrian C. Kuyper, State University of IowaFollow

Document Type

Research

Abstract

The method depends on the specific property of citric acid as a hydrogen donator in accelerating the decolorization of methylene blue by an enzyme found in cucumber seed. It gives reliable results only when enzyme extracts are prepared in the same way and used at the same age. Sodium, calcium and hexose-diphosphate do not interfere unless present in concentrations higher than those found in the blood. Oxalic acid does interfere because it changes the minimum amount of citrate necessary for maximum speed of decolorization. The sensitivity is directly proportional to the amount of methylene blue present in the reaction tube.

Publication Date

1931

Journal Title

Proceedings of the Iowa Academy of Science

Volume

38

Issue

1

First Page

172

Last Page

172

Copyright

©1931 Iowa Academy of Science, Inc.

Language

en

File Format

application/pdf

Recommended Citation

Kuyper, Adrian C. (1931) "The Micro Determination of Citric Acid by the Thunberg Methylene Blue Method," Proceedings of the Iowa Academy of Science, 38(1), 172-172.
Available at: https://scholarworks.uni.edu/pias/vol38/iss1/37