An investigation was carried out to determine the possibilities of applying spectrophotometric methods to the problem of estimating the concentration of hemoglobin in blood. The concentration of a substance in solution can easily be determined from its transmission of some particular wave length, providing the absorption ratio for that wave length is known. A late model direct reading spectrophotometer was used. The method was found to be fairly accurate and simple and should prove valuable in other physical investigations involving the study of spectral transmission curves of various colored substances. Some interesting irregularities in the shape of the spectral transmission curve in one of the absorption bands of oxyhemoglobin were observed. These investigations were carried out under the direction of Professor Charles Sheard in the Section of Physics at the Mayo Clinic, Rochester, Minnesota.
Proceedings of the Iowa Academy of Science
©1927 Iowa Academy of Science, Inc.
Davis, George E.
"A Spectrophotometric Method of Studying Hemoglobin and Other Colored Substances in Solution,"
Proceedings of the Iowa Academy of Science, 34(1), 279-280.
Available at: https://scholarworks.uni.edu/pias/vol34/iss1/82