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Document Type

Research

Abstract

An investigation was carried out to determine the possibilities of applying spectrophotometric methods to the problem of estimating the concentration of hemoglobin in blood. The concentration of a substance in solution can easily be determined from its transmission of some particular wave length, providing the absorption ratio for that wave length is known. A late model direct reading spectrophotometer was used. The method was found to be fairly accurate and simple and should prove valuable in other physical investigations involving the study of spectral transmission curves of various colored substances. Some interesting irregularities in the shape of the spectral transmission curve in one of the absorption bands of oxyhemoglobin were observed. These investigations were carried out under the direction of Professor Charles Sheard in the Section of Physics at the Mayo Clinic, Rochester, Minnesota.

Publication Date

1927

Journal Title

Proceedings of the Iowa Academy of Science

Volume

34

Issue

1

First Page

279

Last Page

280

Copyright

© Copyright 1927 by the Iowa Academy of Science, Inc.

Language

en

File Format

application/pdf

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