The process of amino acid synthesis in plants involves essentially a reduction of nitrates absorbed from the soil. It is difficult, however, to distinguish newly synthesized amino acids from those originating as hydrolytic cleavage products of proteins. To circumvent this difficulty, earlier investigators (2, 5, 6, 8), exposed their plants to nitrogen starvation, thereby reducing soluble nitrogen compounds in tissues to a minimum. Unfortunately, it is extremely difficult to remove final traces of nitrates and ammonia by starvation without causing high mortality in experimental material, and pathological conditions in many of the plants which do survive this rigorous treatment.
Proceedings of the Iowa Academy of Science
©1927 Iowa Academy of Science, Inc.
Loehwing, Walter F.
"Preliminary Report on Amino Acid Synthesis in Plants,"
Proceedings of the Iowa Academy of Science, 34(1), 115-118.
Available at: https://scholarworks.uni.edu/pias/vol34/iss1/26