In the isolation of Bacterium tumefaciens from overgrowths on apple, one of the greatest sources of contamination is that of soil organisms. These are lodged in the soil within the crevices of the convoluted gall surface. In addition the crown gall organisms frequently occur in only small numbers, making it necessary to use large amounts of tissue for isolation purposes.
Proceedings of the Iowa Academy of Science
©1927 Iowa Academy of Science, Inc.
Patel, M. K.
"The use of Sodium Taurocholate and Crystal Violet in the Isolation of Bact. tumefaciens Sm. & Town.,"
Proceedings of the Iowa Academy of Science, 34(1), 38-39.
Available at: https://scholarworks.uni.edu/pias/vol34/iss1/14