In the scientific study of many bacteriological problems it is necessary to deal with pure cultures of the organism with which the worker is interested. This has been true in our studies on the nodule producing organisms of leguminous plants. Before any morphological or physiological studies are made on these organisms they should be tested on the host plant for their inoculating ability, and in order to do this all possible chances for contamination by other organisms must be eliminated. If, in testing out a certain culture for its inoculating ability on the host plant, the plants were grown under conditions that were not sterile, and certain results were obtained, we would have no right to say that these results were due to the culture used, because they may have been due to contamination from the air, from the soil used, or from using seeds that were not sterile. In our studies on these organisms we have attempted to overcome these difficulties and sources of error, and it is the purpose of this paper to present the results of some preliminary work on the sterilization of seeds of the Leguminosae.
Proceedings of the Iowa Academy of Science
©1926 Iowa Academy of Science, Inc.
Walker, Rudger H. and Erdman, Lewis W.
"Preliminary Note on the Sterilization of Seeds of the Leguminosae with Hydrogen Peroxide,"
Proceedings of the Iowa Academy of Science, 33(1), 91-95.
Available at: https://scholarworks.uni.edu/pias/vol33/iss1/10