Honors Program Theses


Open Access Honors Program Thesis

First Advisor

Kirk Manfredi


Water samples were collected in May 2022 from WTH lake and silent lake located in Wind Cave National Park, South Dakota. From the water samples collected, 8 pure fungal colonies were isolated. All samples underwent chemical extraction, growth inhibition testing, proton-nuclear magnetic resonance spectroscopy ( 1H NMR), and high-pressure liquid chromatography (HPLC). Three pure colonies also underwent DNA extraction, and sequencing of the ITS region for genus level identification. These samples were able to be identified to the genus level, and for these three samples were: Talaromyces, sp., Linnemannia, sp., and Gliomatsix, sp. The secondary metabolite that showed antimicrobial activity from Talaromyces, sp. was determined to be flavomannin A. The secondary metabolites of samples were of interest due to the increasing demand for novel compounds obtaining antimicrobial properties for use in the production of new antibiotics to combat the increasing issue of antibiotic resistance.

Year of Submission



Department of Chemistry and Biochemistry

University Honors Designation

A thesis submitted in partial fulfillment of the requirements for the designation University Honors

Date Original


Object Description

1 PDF file (25 pages)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.