Honors Program Thesis (UNI Access Only)
Ira Simet, Honors Thesis Advisor
The process of DNA replication is initiated in eukaryotes by the multisubunit enzyme DNA polymerase ⍺. The relative expression of this enzyme complex plays a role in the amount of DNA replication and cellular reproduction. The transcriptional regulatory mechanisms of the complex are not well known. In this study, chicken liver DNA was extracted, and primer sequences were designed in the preliminary steps to allow for analysis of mRNA concentrations in differently aged chicken embryos. However, difficulties with DNA amplification were not resolved, so quantitative PCR was not performed. Further research needs to be done to obtain the proper conditions for successful DNA amplification, which will provide the basis for more exploration using quantitative PCR.
Year of Submission
Department of Chemistry and Biochemistry
University Honors Designation
A thesis submitted in partial fulfillment of the requirements for the designation University Honors
1 PDF file (10 pages)
©2021 Carter Howard Stochi
Stochl, Carter Howard, "Quantitative analysis of DNA polymerase alpha gene expression during various stages in chicken embryonic development" (2021). Honors Program Theses. 494.