Open Access Honors Program Thesis
Justin Peters, Honors Thesis Advisor
DNA--Structure; Capillary electrophoresis;
A-tracts are sequences of repeated adenine bases that cause a natural curvature of DNA. The biological function of A-tracts is not fully understood. Research into how A-tract sequences affect mobility in an electric field contributes more knowledge in this area. For this research project, the electrophoretic mobility of multiple double-stranded DNA (dsDNA) fragments of the same length, with varying shapes due to the amount and orientation of A-tracts along the helix axis, was studied using capillary electrophoresis under varying conditions with the small molecule netropsin. Previous semesters of research showed a change in mobility due to shape of three fragments that contain four A-tracts in different orientations. Netropsin conditions were of interest in regards to mobility changes because this small molecule is known to bind the minor groove of A-tracts. Netropsin binding was found to eliminate the mobility difference of the different dsDNA fragments in a concentration-dependent manner.
Year of Submission
Department of Chemistry and Biochemistry
University Honors Designation
A thesis submitted in partial fulfillment of the requirements for the designation University Honors
1 PDF file (13 pages)
©2021 Jillian Miller
Miller, Jillian, "Examining the effects of netropsin on the mobility of DNA A-tracts using capillary electrophoresis" (2021). Honors Program Theses. 479.