Open Access Honors Program Thesis
Justin Peters, Honors Thesis Advisor
Ali Tabei, Honors Thesis Advisor
DNA--Conformation; Protein binding;
This project used Atomic Force Microscopy (AFM) imaging to examine the effects of different intercalation conditions upon the elastic properties of intrinsically straight DNA molecules. Three intercalating molecules (chloroquine, ethidium bromide, and acridine) were used to demonstrate the dependence of persistence length and mean polymer extension on the intercalator type. It was found that all three intercalators significantly increased persistence length, and that this effect was most pronounced for ethidium bromide. In addition, an analysis of the normal bending modes of the static molecules corroborated these results. The only intercalator which resulted in significant extension of the DNA polymers was ethidium bromide. By measuring these properties for a model system of DNA, the understanding of binding effects of intercalators and the bending properties of generic DNA molecules will be improved.
Year of Submission
Department of Chemistry and Biochemistry
University Honors Designation
A thesis submitted in partial fulfillment of the requirements for the designation University Honors
1 PDF file (22 pages)
©2020 Joseph Tibbs
Tibbs, Joseph, "The effects of intercalating molecules on the polymer properties of DNA" (2020). Honors Program Theses. 435.